Antimicrobial activity of citronella oil

Finally, LA at high concentration was genotoxic to peripheral human lymphocytes 54 and to human monocyte THP-1 cells In the present study, CL EO, which is known for its high eugenol 41 content a phenolic compound was not found to be the most active EO against the microorganisms tested in the disc-diffusion assay.

CL also had no fungicidal activity against A. Introduction Essential oils EOs are products derived from aromatic plants which contain around 20—60 components at quite different concentrations 1. For example several Gram-negative and Gram-positive bacteria are sensitive to various EOs 23141516showing clear zones on agar assays in which the tested EO inhibits the growth of a particular microorganism.

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The effects of a 24 h treatment of different concentrations of EOs 0. Bacterial isolates A total of 36 bacterial isolates See Table 1 from 10 different species of aquatic animals Penaeus vannamei, Penaeus monodon, Macrobrachium rosenbergii, Scylla sp. Therefore, this study was carried out to reveal the chemical composition and potential of C. The resulting increase in human exposure as a consequence of this expanded usage therefore requires a careful re-assessment of their toxicity and genotoxicity on the level of mammalian cells In the present study, CL EO, which is known for its high eugenol 41 content a phenolic compound was not found to be the most active EO against the microorganisms tested in the disc-diffusion assay. Clove EO from Eugenia caryophyllata L. The components of the test solution were identified by comparing the spectra with those of known compounds stored in internal library. EOs with biocidal activity were used to develop alternative disinfection strategies for indoor environments or in the food industry, on contaminated surfaces and equipment in food processing environments 15 , 24 , 25 , It should be noted that the disc-diffusion method is limited by the hydrophobic nature of most EOs, which prevents their uniform diffusion through the agar medium. On the other hand, it has also been shown that high concentrations of some EOs contributed to harmful changes in the body Some studies also determined the minimal inhibition and minimal bactericidal concentrations in liquid medium 11 ,

Environmental bacterial strains were much more sensitive to chloramphenicol than clinical strains; no significant difference in susceptibility was found between Gram-negative and Gram-positive bacteria.

Myrtaceae has shown antibacterial, antifungal, anti-oxidant 8 and anti-inflammatory effects 9. Although EOs were applied in the past to successfully treat a variety of diseases and to preserve health, they have been used more frequently for a greater variety of applications in recent years, including drugs, crop protectants, food additives, aromatherapy, and others.

citronella oil extraction pdf

The effects of a 24 h treatment of different concentrations of EOs 0. The greatest antifungal activity against all tested strains was exhibited by OR, which had MICs of 0.

Some studies have reported that EOs tend to act more strongly on Gram-positive than Gram-negative bacteria 23444presumably due to differences in cell wall composition All four EOs inhibited the growth of both clinical and environmental Gram-positive S.

Citronella oil components

However, EOs can also exist in a potentially highly bioactive vapor phase, and some EOs have shown antimicrobial activity that does not require direct contact with the EO 18 , 19 , 20 , Figure 5 Cytotoxicity or viability of human HEL cells. On the other hand, it has also been shown that high concentrations of some EOs contributed to harmful changes in the body We have demonstrated the EOs do have cytotoxic effect, but only at higher concentrations on HEL human embryonic lung cells under in vitro conditions. This article has been cited by other articles in PMC. Oregano, thyme, clove and arborvitae showed very strong antibacterial activity against all tested strains at both full strength and reduced concentrations. Minimum inhibitory concentration MIC of the essential oil tested against bacterial isolates from various aquatic animals and ATCC type strains were determined using two-fold broth micro dilution method with kanamycin and eugenol as positive controls.

EOs have shown antitumor activity both in vitro and in vivo and low toxicity 50 ,

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